Biotyping of Clostridium Perfringens strains isolated from enterotoxemia cases in sheep using ELISA technique

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Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. Th e purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence o...

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Prevalence and Toxinotyping of the Toxigenic Clostridium perfringens in Sheep with suspected Enterotoxemia

The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in AlAhsa province, KSA. Out of 240 intestinal content samples collected from dead sheep with suspected enterotoxemia, 34 Clostridial strains were isolated. Diagnosis was based on classical diagnostic tests, enzyme-linked immunosorbent assay (ELISA) and multiplex polym...

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ژورنال

عنوان ژورنال: Iraqi Journal of Veterinary Sciences

سال: 2010

ISSN: 2071-1255

DOI: 10.33899/ijvs.2010.5583